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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of France, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.
목차
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참고문헌 (15)
참고문헌 신청
Alasina, M. and A. R. Blanch. 1994. Improvement and update of a set of keys for biochemical identification of Vibrio species. J. Appl. Bacteriol., 77, 719-721.
Allam, B., C. Paillard, and S. E. Ford. 2002. Pathogenicity of Vibrio tapetis, the etiologic agent brown ring disease in clams. Dis. Aquat. Org. 48, 221-231.
Castro, D., J. A. Santamaria, A. Luque, E. Marinez-Manzanares, and J. J. Borrego. 1996. Antigenic characterization of the etiological agent of the brown ring disease affecting manila clams. Syst. Appl. Microbiol., 19, 231-239.
Elliot, E. L., C. A. Kaysner and M. L. Tamplin. 1992. Vibrio parahaemolyticus, V. cholerae, V. vulnificus and other Vibrio spp. In Food and Drug Administration. Bacteriological Analytical Manual, 7th ed. AOAC International, Arlington. pp. 111-140.
Euzeby, J. P. 1997. List of bacterial names with standing in nomenclature: a folder available on the Internet (URL:http://www.bacterio.cict.fr/). Int. J. Syst. Bacteriol. 47, 590-592.
Allam, B., C. Paillard, and S. E. Ford. 2002. Pathogenicity of Vibrio tapetis, the etiologic agent brown ring disease in clams. Dis. Aquat. Org. 48, 221-231.
Castro, D., J. A. Santamaria, A. Luque, E. Marinez-Manzanares, and J. J. Borrego. 1996. Antigenic characterization of the etiological agent of the brown ring disease affecting manila clams. Syst. Appl. Microbiol., 19, 231-239.
Elliot, E. L., C. A. Kaysner and M. L. Tamplin. 1992. Vibrio parahaemolyticus, V. cholerae, V. vulnificus and other Vibrio spp. In Food and Drug Administration. Bacteriological Analytical Manual, 7th ed. AOAC International, Arlington. pp. 111-140.
Euzeby, J. P. 1997. List of bacterial names with standing in nomenclature: a folder available on the Internet (URL:http://www.bacterio.cict.fr/). Int. J. Syst. Bacteriol. 47, 590-592.
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UCI(KEPA) : I410-ECN-0101-2009-470-017681419