지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: We performed simultaneous determination of five constituents by HPLC in Samul-tang (SMT). Additionally, we investigated the immune-stimulatory potential of SMT on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice.
Methods: Reverse-phase chromatography using a Gemini C18 column operating at 40°C, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 ㎍/200 ㎍) on days 1, 8, and 15. The extract of SMT (1000 ㎎/㎏) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma.
Results: Calibration curves were acquired with r2＞0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 ㎎/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p＜0.01). Also, SMT significantly enhanced OVAspecific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group.
Conclusions: The established method will be applied for the quantification of major components and immunestimulating activity in OVA-immunized mouse model of SMT.
Methods: Reverse-phase chromatography using a Gemini C18 column operating at 40°C, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 ㎍/200 ㎍) on days 1, 8, and 15. The extract of SMT (1000 ㎎/㎏) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma.
Results: Calibration curves were acquired with r2＞0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 ㎎/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p＜0.01). Also, SMT significantly enhanced OVAspecific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group.
Conclusions: The established method will be applied for the quantification of major components and immunestimulating activity in OVA-immunized mouse model of SMT.
목차
Introduction
Materials and Methods
Results
Discussion
Conclusion
Acknowledgments
References
참고문헌 (16)
참고문헌 신청
1. [학술지(정기간행물)] - Chang-Seob Seo / 2010 / Simultaneous Determination of Albiflorin, Cinnamaldehyde, Cinnamic Acid, Daidzin, Glycyrrhizin, Liquiritin, Paeoniflorin and Puerarin in Galgeun-tang by HPLC-PDA / 대한한의학회지 31 (6) : 8 ~ 15
2. [학술지(정기간행물)] - Jin Bae Won / 2010 / Simultaneous Determination of Four Marker Components in Yukmijihwang Tang by High Performance Liquid Chromatography/Diode Array Detector / Archives of Pharmacal Research 33 (4) : 619 ~ 623
3. [학술지(정기간행물)] - Zhang H / 2004 / Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESI-MS / J Pharm Biomed Anal 34 : 705 ~ 713
4. [학술지(정기간행물)] - Jiang WY / 2005 / Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science / Trends Pharmacol Sci 26 : 558 ~ 563
5. [학술지(정기간행물)] - Liu S / 2008 / Traditional Chinese medicine and separation science / J Sep Sci 31 : 2113 ~ 2137
2. [학술지(정기간행물)] - Jin Bae Won / 2010 / Simultaneous Determination of Four Marker Components in Yukmijihwang Tang by High Performance Liquid Chromatography/Diode Array Detector / Archives of Pharmacal Research 33 (4) : 619 ~ 623
3. [학술지(정기간행물)] - Zhang H / 2004 / Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESI-MS / J Pharm Biomed Anal 34 : 705 ~ 713
4. [학술지(정기간행물)] - Jiang WY / 2005 / Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science / Trends Pharmacol Sci 26 : 558 ~ 563
5. [학술지(정기간행물)] - Liu S / 2008 / Traditional Chinese medicine and separation science / J Sep Sci 31 : 2113 ~ 2137
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UCI(KEPA) : I410-ECN-0101-2014-519-000281295