The aim of this study is measurement of low back muscle fatigue in long term driving. Recently studies show that muscle fatigue using EMG was measured during long-term driving. However results have no correlation between muscle fatigue and back pain. For these reasons, a study measures to muscle characteristic using by MyotonPro. Result shows that Creep and Relaxation was increased at right side of low back, Frequency and Stiffness increased at left side of low back. These results shows that low level contraction evoked by long-term driving would be measured using MyotonPRO.