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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
Tae-Yun Lee (Yeungnam University) Yu-Kyoung Park (Keimyung University) Byeong-Churl Jang (Keimyung University)
저널정보
계명대학교 자연과학연구소 Quantitative Bio-Science Quantitative Bio-Science Vol.37 No.1
발행연도
2018.5
수록면
57 - 63 (7page)

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Adipogenesis, also called the differentiation of preadipocytes, leads to the phenotype of mature adipocytes. However, excessive adipogenesis is closely linked to the development of obesity. Cyclosporin A, a neutral lipophilic cyclic undecapeptide isolated from the fungus Hypocladium inflatum gams, is known for several biological activities, including immunosuppression and anti-inflammation. Little is known about the relationship between cyclosporin A and obesity. In this study, we investigated the effect of cyclosporin A on adipogenesis in 3T3-L1 murine preadipocytes. 3T3-L1 preadipocyte differentiation was evaluated by Oil Red O staining and AdipoRed assay. The expression and phosphorylation levels of adipogenesis-related proteins in differentiating 3T3-L1 cells were evaluated by Western blotting. The mRNA expression levels of adipogenesis-related proteins in differentiating 3T3-L1 cells were measured by RT-PCR. Cyclosporin A considerably inhibited lipid accumulation and reduced triglyceride (TG) contents during the differentiation of 3T3-L1 preadipocytes into adipocytes in a concentration-dependent manner, suggesting its anti-adipogenic effect. Treatment with cyclosporin A at the concentrations tested was not cytotoxic to 3T3-L1 cells. Mechanistically, at a 10 μM concentration, cyclosporin A strongly down-regulated the protein and mRNA expressions of two adipogenic transcription factors, CCAAT/enhancer-binding protein-α (C/EBP-α) and peroxisome proliferator-activated receptor-γ (PPAR-γ) in differentiating 3T3-L1 cells. Furthermore, at a 10 μM concentration, cyclosporin A reduced the phosphorylation levels of signal transducers and activator of transcription-3 (STAT-3), other adipogenic transcription factor, and suppressed the protein and mRNA expressions of perilipin A, a protein that binds to and stabilizes lipid droplets, in differentiating 3T3-L1 cells. However, treatment with 10 μM of cyclosporin A did not modulate the protein and mRNA expressions of fatty acid synthase during 3T3-L1 preadipocyte differentiation. Collectively, these results show that cyclosporin A inhibits adipogenesis in differentiating 3T3-L1 cells, and that this inhibition is mediated through the reduced expression and phosphorylation levels of C/EBP-α, PPAR-γ, perilipin A, and STAT-3.
#Cyclosporin A #Adipogenesis #C/EBP-α #PPAR-γ #3T3-L1

목차

Abstract
Introduction
Materials and Methods
Results
Discussion
References

참고문헌 (25)

참고문헌 신청
[학술지(정기간행물)] - Kopelman PG / 2000 / Obesity as a medical problem / Nature 404 : 635 ~ 643 Crossref [학술지(정기간행물)] - Ahima RS / 2000 / Adipose tissue as an endocrine organ / Trends Endocrinol Metab 11 : 327 ~ 332 Crossref [학술지(정기간행물)] - Havel PJ / 2002 / Control of energy homeostasis and insulin action by adipocyte hormones: leptin acylation stimulating protein, and adiponectin / Curr Opin Lipidol 13 : 51 ~ 59 Crossref [학술지(정기간행물)] - Neal JW / 2002 / Calcineurin mediates the Calciumdependent inhibition of adipocyte differentiation in 3T3-L1 cells / J Biol Chem 277 : 49776 ~ 49781 google schola [학술지(정기간행물)] - Spiegelman BM / 2001 / Obesity and the regulation of energy balance / Cell 104 : 531 ~ 543 Crossref

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UCI(KEPA) : I410-ECN-0101-2018-047-002155039