지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.8
- 수록면
- 247 - 258 (12page)
- DOI
- 10.7783/KJMCS.2019.27.4.247
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초록· 키워드
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Background: Astragali radix (A) and Lithospermi radix (L) have long been used as traditional medicines due to their known anti-inflammatory effects. This study aimed at evaluating, their optimal mixing ratio and their functional compounds by investigating the inhibitory effects of mixed extracts of A and L and their active compounds on matrix metalloproteinases (MMPs).
Methods and Results: A and L extracts were obtained by extraction at 80℃ using 50% and 70% fermented alcohol, respectively, and then mixed at a ratio of 5 : 5, 6 : 4, 7 : 3 and 8 : 2 (w/w). The activities of MMP-1, MMP-3, and MMP-13 were evaluated in interleukin-1beta (IL-1β)-induced SW1353 cells. The extract mixtures showed synergistic inhibitory effects on MMP-3 and MMP-13, higher than the effects of the individual A and L extracts. The 7 : 3 mixture (ALM16) showed the most effective MMPs inhibitory activity, while among the active ingredients, calycosin-7-O-β-D-glucoside and lithospermic acid exhibited excellent MMPs inhibitory activity. Additionally, an HPLC method was established for simultaneous quantification of the effective components of the extract mixtures, and validated by measuring the linearity, precision and accuracy of the limit of detection (LOD) and limit of quantification (LOQ).
Conclusions: ALM16 showed the most effective MMPs inhibitory activity. Calycosin-O-β-D-glucoside, calycosin and lithospermic acid were identified as useful candidates, as they were the major functional compounds in the MMP inhibitory activity. Summarily, ALM16 might be a highly effective in osteoarthritis management, owing to its because it exhibits a protective effect on cartilage via excellent inhibition of MMPs.
Methods and Results: A and L extracts were obtained by extraction at 80℃ using 50% and 70% fermented alcohol, respectively, and then mixed at a ratio of 5 : 5, 6 : 4, 7 : 3 and 8 : 2 (w/w). The activities of MMP-1, MMP-3, and MMP-13 were evaluated in interleukin-1beta (IL-1β)-induced SW1353 cells. The extract mixtures showed synergistic inhibitory effects on MMP-3 and MMP-13, higher than the effects of the individual A and L extracts. The 7 : 3 mixture (ALM16) showed the most effective MMPs inhibitory activity, while among the active ingredients, calycosin-7-O-β-D-glucoside and lithospermic acid exhibited excellent MMPs inhibitory activity. Additionally, an HPLC method was established for simultaneous quantification of the effective components of the extract mixtures, and validated by measuring the linearity, precision and accuracy of the limit of detection (LOD) and limit of quantification (LOQ).
Conclusions: ALM16 showed the most effective MMPs inhibitory activity. Calycosin-O-β-D-glucoside, calycosin and lithospermic acid were identified as useful candidates, as they were the major functional compounds in the MMP inhibitory activity. Summarily, ALM16 might be a highly effective in osteoarthritis management, owing to its because it exhibits a protective effect on cartilage via excellent inhibition of MMPs.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (45)
참고문헌 신청
Aigner T / 2002 / Molecular pathology and pathobiology of osteoarthritic cartilage / Cellular and Molecular Life Sciences 59:5~18
Blaney Davidson EN / 2009 / Increase in ALK1/ALK5 ratio as a cause for elevated MMP-13 expression in osteoarthritis in humans and mice / Journal of Immunology 182:7937~7945
Chan KWK / 2015 / Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachlorideinduced liver oxidative damage in vitro and in vivo / Oncology Reports 34:673~680
Chen JJ / 2014 / Expression and significance of MMP3 in synovium of knee joint at different stage in osteoarthritis patients / Asian Pacific Journal of Tropical Medicine 7:297~300
Cho WCS / 2007 / In vitro and in vivo anti-tumor effects of Astragalus membranaceus / Cancer Letters 252:43~54
Blaney Davidson EN / 2009 / Increase in ALK1/ALK5 ratio as a cause for elevated MMP-13 expression in osteoarthritis in humans and mice / Journal of Immunology 182:7937~7945
Chan KWK / 2015 / Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachlorideinduced liver oxidative damage in vitro and in vivo / Oncology Reports 34:673~680
Chen JJ / 2014 / Expression and significance of MMP3 in synovium of knee joint at different stage in osteoarthritis patients / Asian Pacific Journal of Tropical Medicine 7:297~300
Cho WCS / 2007 / In vitro and in vivo anti-tumor effects of Astragalus membranaceus / Cancer Letters 252:43~54
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