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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 161 - 168 (8page)
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초록· 키워드
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Genotyping-by-sequencing (GBS) is a cost-efficient method which can be useful for SNP marker discovery in a population of interest. GBS is genome reduction sequencing method using restriction enzyme. The quality of DNA is a key factor which could have an influence in downstream analysis. However, there have not been many studies which investigated the impact of DNA degradation and the quality of the data on marker discovery. In this study, GBS data of 6 Hanwoo samples (H1~6) showing differing level of DNA degradation were compared. Re-sequencing pipeline was followed to investigate the impact of DNA degradation on marker discovery. As a result, we found that the quantity and quality of SNPs were not affected in the sample H5 and H6 with moderately degraded DNA. On the other hand, marker discovery was greatly affected in samples with severe DNA degradation (H3 and H4). The findings in this study support that GBS is a robust genotyping method towards moderate DNA degradation.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Bolger, A. M. / 2014 / Trimmomatic : a flexible trimmer for Illumina sequence data / Bioinformatics 30 (15) : 2114 ~ 2120
Brotherton, P. / 2007 / Novel high-resolution characterization of ancient DNA reveals C> U-type base modification events as the sole cause of post mortem miscoding lesions
Bruskov, V. I. / 2002 / Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA / Nucleic Acids Res 30 (6) : 1354 ~ 1363
Cadet, J. / 2005 / Ultraviolet radiation-mediated damage to cellular DNA / Mutat. Res 571 (1) : 3 ~ 17
De Donato, M. / 2013 / Genotyping-bysequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing / PLoS One 8 (5) : e
Brotherton, P. / 2007 / Novel high-resolution characterization of ancient DNA reveals C> U-type base modification events as the sole cause of post mortem miscoding lesions
Bruskov, V. I. / 2002 / Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA / Nucleic Acids Res 30 (6) : 1354 ~ 1363
Cadet, J. / 2005 / Ultraviolet radiation-mediated damage to cellular DNA / Mutat. Res 571 (1) : 3 ~ 17
De Donato, M. / 2013 / Genotyping-bysequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing / PLoS One 8 (5) : e
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