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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 6 (6page)
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Although soft tunic syndrome (STS) in the ascidian is a serious disease, helpful measures have yet not been established. It was examined in this study by applying aniti-parasitic drugs to eradicate the causative protozoa Azumiobodo hoyamushi from infected ascidians. Formalin was synergistic in killing parasites in vitro when co-treated with hydrogen peroxide (H2O2) or bronopol, but not with chloramine-T or povidone-iodine (PVP-I), when tested with in vitro parasite culture. The synergistic effects did not change when formalin-H2O2 (or bronopol) ratios were changed.
It was found that treatment periods less than 60 min achieved a sub-maximal efficacy. Increasing drug concentration while keeping 30 min period improved anti-parasitic effects. Anti-parasitic effects of formalin(F) + H2O2(H) were also assessed in an in vivo STS model infected with cultured parasites. It was observed that combined 50 (40F + 10H) and 100 (80F +20H) ppm were effective in partially preventing STS-caused mortality. In horizontally transmitted artificial STS model, significant prevention of ascidian mortality was also observed after 50 ppm. Marked reduction of living parasites were noted after drug treatments in vivo. The results provide a highly useful basis to develop a preventive or treatment measure against the currently uncontrollable STS in the ascidian.
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참고문헌 (23)
참고문헌 신청
Bell A / 2005 / Antimalarial drug synergism and antagonism : mechanism and clinical significance / FEMS Microbiol Lett 253:171~184
Bower SM / 1989 / Disinfectants and therapeutic agents for controlling Labyrinthuloides haliotidis(Protozoa : Labyrinthomorpha), an abalone pathogen / Aquculture 78:207~215
Cho HK / 2008 / Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi / Mar Biotechnol 10:447~456
Gilbert JP / 1979 / An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system / J Fish Dis 2:191~196
Harms CA / 1996 / Treatments for parasitic diseases of aquarium and ornamental fish / Sem Avian Exot Pet Med 5:54~63
Bower SM / 1989 / Disinfectants and therapeutic agents for controlling Labyrinthuloides haliotidis(Protozoa : Labyrinthomorpha), an abalone pathogen / Aquculture 78:207~215
Cho HK / 2008 / Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi / Mar Biotechnol 10:447~456
Gilbert JP / 1979 / An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system / J Fish Dis 2:191~196
Harms CA / 1996 / Treatments for parasitic diseases of aquarium and ornamental fish / Sem Avian Exot Pet Med 5:54~63
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