지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 81 - 88 (8page)
이용수
초록· 키워드
오류제보하기
We investigated factors affecting primary cultures of Pacific abalone Haliotis discus hannai ovary-dissociated cells to identify general aspects of their early-phase culture. Ninety-seven cell populations derived from 30 individuals were cultured in different media with varying compositions of medium supplements, and initial attachment, subculture, and survival for ≥10 weeks were assessed according to medium composition and individual. We also examined the time required for subculture and the rate of cell death according to both culturing period and passage number within 10 weeks. A lack of fetal bovine serum (FBS) and hemolymph significantly inhibited the growth of cultured cells, while we detected no significant effect of medium composition on initial cell attachment. Through data reallocation, with the omission of data from cell populations cultured in FBS-free and hemolymph-free media, we showed that growth inhibition was also affected by individual differences among the abalones used. During the culture, we observed four different types of cell morphology. Moreover, considerable time was required for subculture—18.4 and 19.5 days for first and second subcultures, respectively—and cell death did not occur within 30 days or for passage 0. Our results will provide valuable information for developing universal cell culturing guidelines in abalone species and suggest the feasibility of culturing abalone ovary-dissociated cells.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Andrae J / 2008 / Role of platelet-derived growth factors in physiology and medicine / Genes Dev 22 : 1276 ~ 1312
Auzoux-Bordenave S / 2007 / CGRP regulates the activity of mantle cells and hemocytes in abalone primary cell cultures(Haliotis tuberculata) / J Shellfish Res 26 : 887 ~ 894
Awaji M / 2004 / Gonad formation, sex differentiation and gonad maturation processes in artificially produced juveniles of the abalone, Haliotis discus hannai / Aquaculture 2
Chen SN / 1999 / Establishment of cell lines derived from oyster, Crassostrea gigas Thunberg and hard clam, Meretrix lusoria Roding / Meth Cell Sci 21 : 183 ~ 192
Choi SS / 2005 / Beneficial effect of silkworm hemolymph on a CHO cell system : Inhibition of apoptosis and increase of EPO production / Biotechnol Bioeng 91 : 793 ~ 800
Auzoux-Bordenave S / 2007 / CGRP regulates the activity of mantle cells and hemocytes in abalone primary cell cultures(Haliotis tuberculata) / J Shellfish Res 26 : 887 ~ 894
Awaji M / 2004 / Gonad formation, sex differentiation and gonad maturation processes in artificially produced juveniles of the abalone, Haliotis discus hannai / Aquaculture 2
Chen SN / 1999 / Establishment of cell lines derived from oyster, Crassostrea gigas Thunberg and hard clam, Meretrix lusoria Roding / Meth Cell Sci 21 : 183 ~ 192
Choi SS / 2005 / Beneficial effect of silkworm hemolymph on a CHO cell system : Inhibition of apoptosis and increase of EPO production / Biotechnol Bioeng 91 : 793 ~ 800
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli
Fisheries and Aquatic Sciences
2016 .01
-
Intestinal Microbiota in Pacific abalone Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2017 .05
-
Investigation of the intestinal microbiota of Pacific abalone, Haliotis discus hannai, with differences in growth performance
한국수산과학회 양식분과 학술대회
2020 .07
-
Effects of Temperature on Survival and Growth of Three Juvenile Abalone Haliotis sieboldii, Haliotis discus and Haliotis discus hannai
수산해양교육연구
2018 .08
-
Efficient method for the preparation of metaphase chromosomes through mantle tissue culture in adult abalone Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2018 .05
-
Molecular characterization and expression of the Macrophage migration inhibitory factor gene from the pacific abalone (Haliotis discus hannai)
한국수산과학회 양식분과 학술대회
2017 .05
-
Transcriptome analysis of induced triploid abalone (Haliotis discus hannai) tissues
한국수산과학회 양식분과 학술대회
2016 .10
-
ANALYSIS OF GENES ASSOCIATED WITH THE GROWTH OF Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2015 .05
-
Pathological studies on exfoliation and recovery in oxidative stress-induced abalone, Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2016 .10
-
Signaling systems of gonadotropin-releasing hormone related peptides in Pacific abalone, Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2022 .10
-
Histological analysis of gonads in two-year-old triploid abalone (Haliotis discus hannai)
한국수산과학회 양식분과 학술대회
2016 .10
-
Differentially expressed abalone (Haliotis discus hannai) transcripts during experimental exposure to LPS and Cu
한국수산과학회 양식분과 학술대회
2016 .10
-
Effects of the addition of Sargassum horneri in feed on growth and gut microbiota of abalone, Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2022 .10
-
Identification and expression profiles of gonadotropin-releasing hormone messenger RNA in the Pacific abalone Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2017 .05
-
인공어초 형상과 재질 및 수온에 따른 전복(Haliotis discus hannai, abalone)의 선호도
어업기술연구소보고
2020 .02
-
Isolation of Anti-Inflammatory Compounds from Abalone (Haliotis discus hannai) Viscera via NO Production-Guided Fractionation in LPS-Induced Murine Macrophage, RAW 264.7 Cells
한국식품영양과학회 학술대회발표집
2020 .10
-
Differential expression of hypoxia-inducible genes during experimentally designed stimulatory treatments in abalone Haliotis discus hannai
한국수산과학회 양식분과 학술대회
2015 .10
-
Molecular characterization of the male-specific predominant gene, Tssk1, from the Pacific abalone (Haliotis discus hannai)
한국수산과학회 양식분과 학술대회
2016 .10
-
Anti-allergic effects of a novel peptide isolated from abalone (Haliotis discus hannai) intestine gastrointestinal digests in activated HMC-1 human mast cell line
한국수산과학회 양식분과 학술대회
2015 .10
-
Hybridization between Haliotis discus hannai and H. discus discus and growth analysis
한국수산과학회 양식분과 학술대회
2016 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0