지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
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- 1 - 9 (9page)
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Cysteine and aspartic proteases possess high elastolytic activity and might contribute to the degradation of the abdominal aortic aneurysm (AAA) wall. The aim of this study was to analyze, in detail, the proteases (cathepsins B, D, K, L and S, and inhibitor cystatin C) found in human AAA and healthy aortic tissue samples. The vessel walls from AAA patients (n¼36) and nonaneurysmal aortae (n¼10) were retrieved using conventional surgical repair and autopsy methods. Serum samples from the same AAA patients and 10 healthy volunteers were also collected. Quantitative expression analyses were performed at the mRNA level using real-time reverse transcriptase-PCR (RT–PCR). Furthermore, analyses at the protein level included western blot and immunoprecipitation analyses. Cellular sources of cysteine/aspartic proteases and cystatin C were identified by immunohistochemistry (IHC). All cysteine/aspartic proteases and cystatin C were detected in the AAA and control samples.Using quantitative RT–PCR, a significant increase in expression was observed for cathepsins B (P¼0.021) and L (P¼0.018),compared with the controls. Cathepsin B and cystatin C were also detected in the serum of AAA patients. Using IHC, smooth muscle cells (SMCs) and macrophages were positive for all of the tested cathepsins, as well as cystatin C; in addition, the lymphocytes were mainly positive for cathepsin B, followed by cathepsins D and S. All cysteine/aspartic proteases analyzed in our study were detected in the AAA and healthy aorta. The highest expression was found in macrophages and SMCs. Consequently, cysteine/aspartic proteases might play a substantial role in AAA.
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참고문헌 (25)
참고문헌 신청
Abdul-Hussien H / 2007 / Collagen degradation in the abdominal aneurysm : a conspiracy of matrix metalloproteinase and cysteine collagenases / Am J Pathol 170 : 809 ~ 817
Abisi S / 2007 / Cysteine protease activity in the wall of abdominal aortic aneurysms / J Vasc Surg 46 : 1260 ~ 1266
Ailawadi G / 2003 / Current concepts in the pathogenesis of abdominal aortic aneurysm / J Vasc Surg 38 : 584 ~ 588
Brewster DC / 2003 / Guidelines for the treatment of abdominal aortic aneurysms. Report of a subcommittee of the Joint Council of the American Association for Vascular Surger
Collin J / 1999 / UK small aneurysms trial / Lancet 353 : 407 ~ 408
Abisi S / 2007 / Cysteine protease activity in the wall of abdominal aortic aneurysms / J Vasc Surg 46 : 1260 ~ 1266
Ailawadi G / 2003 / Current concepts in the pathogenesis of abdominal aortic aneurysm / J Vasc Surg 38 : 584 ~ 588
Brewster DC / 2003 / Guidelines for the treatment of abdominal aortic aneurysms. Report of a subcommittee of the Joint Council of the American Association for Vascular Surger
Collin J / 1999 / UK small aneurysms trial / Lancet 353 : 407 ~ 408
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