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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 216 - 222 (7page)
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Growth factor stimulation induces Y783 phosphorylation of phosphoinositide-specific PLC-γ1, and the subsequent activation of this enzyme in a cellular signaling cascade. Previously, we showed that a double point mutation, Y509A/F510A, of PLC-γ1, abolished interactions with translational elongation factor 1-α.
Here, we report that the Y509A/F510A mutant PLC-γ1displayed extremely high levels of Y783 phosphorylation and enhanced catalytic activity, compared to wild-type PLC-γ1, upon treatment of COS7 cells with EGF. In quiescent COS7 cells, the Y509A/F510A mutant PLC-γ1 exhibited a constitutive hydrolytic activity,whereas the wild-type counterpart displayed a basal level of activity. Upon treatment of COS7 cells with EGF, the Y783F mutation in Y509A/F510A PLC-γ1(Y509A/F510A/Y783F triple mutant) cells also led to an enhanced catalytic activity, whereas Y783F mutation alone displayed a basal level of activity. Our results collectively suggest that the Y509A/F510A mutant is more susceptible to receptor tyrosine kinase-induced Y783phosphorylation than is wild-type PLC-γ1, but no longer requires Y783 phosphorylation step for the Y509A/F510A mutant PLC-γ1 activation in vivo.
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참고문헌 (22)
참고문헌 신청
Bae SS / 2002 / Regulation of phospholipase C-gamma1 by protein kinase A-dependent phosphorylation / Adv Enzyme Regul 42 : 195 ~ 211
Chang J-S / 1997 / Overexpression of phospholipase C-gamma1 in rat 3Y1 fibroblast cells leads to malignant transformation / Cancer Res 57 : 5465 ~ 5468
Chang J-S / 2002 / Interaction of elongation factor-1alpha and pleckstrin homology domain of phospholipase C-gamma 1 with activating its activity / J Biol Chem 277 : 19697 ~
Chang J-S / 2005 / Pleckstrin homology domains of phospholipase C-gamma1 directly interact with beta-tubulin for activation of phospholipase C-gamma1 and reciprocal modulatio
Choi JH / 2006 / Phospholipase Cgamma1 negatively regulates growth hormone signalling by forming a ternary complex with Jak2 and protein tyrosine phosphatase-1B / Nat Cell Bi
Chang J-S / 1997 / Overexpression of phospholipase C-gamma1 in rat 3Y1 fibroblast cells leads to malignant transformation / Cancer Res 57 : 5465 ~ 5468
Chang J-S / 2002 / Interaction of elongation factor-1alpha and pleckstrin homology domain of phospholipase C-gamma 1 with activating its activity / J Biol Chem 277 : 19697 ~
Chang J-S / 2005 / Pleckstrin homology domains of phospholipase C-gamma1 directly interact with beta-tubulin for activation of phospholipase C-gamma1 and reciprocal modulatio
Choi JH / 2006 / Phospholipase Cgamma1 negatively regulates growth hormone signalling by forming a ternary complex with Jak2 and protein tyrosine phosphatase-1B / Nat Cell Bi
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