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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제1호
- 발행연도
- 2017.1
- 수록면
- 197 - 205 (9page)
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초록· 키워드
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Exposure of Jurkat T cell clone (J/Neo cells) to acacetin (5,7-dihydroxy-4’-methoxyflavone), which is present in barnyard millet (Echinochloa esculenta (A. Braun)) grains, caused cytotoxicity, enhancement of apoptotic sub-G1 rate, Bak activation, loss of mitochondrial membrane potential (Δψm), activation of caspase-9 and caspase-3, degradation of poly(ADPribose) polymerase, and FITC-Annexin V-stainable phosphatidylserine exposure on the external surface of the cytoplasmic membrane without accompanying necrosis. These apoptotic responses were abrogated in Jurkat T cell clone (J/Bcl-xL) overexpressing Bcl-xL. Under the same conditions, cellular autophagic responses, including suppression of the AktmTOR pathway and p62/SQSTM1 down-regulation, were commonly detected in J/Neo and J/Bcl-xL cells; however, formation of acridine orange-stainable acidic vascular organelles, LC3-I/II conversion, and Beclin-1 phosphorylation (Ser-15) were detected only in J/Neo cells. Correspondingly, concomitant treatment with the autophagy inhibitor (3-methyladenine or LY294002) appeared to enhance acacetin-induced apoptotic responses, such as Bak activation, Δψm loss, activation of caspase-9 and caspase-3, and apoptotic sub-G1 accumulation. This indicated that acacetin could induce apoptosis and cytoprotective autophagy in Jurkat T cells simultaneously. Together, these results demonstrate that acacetin induces not only apoptotic cell death via activation of Bak, loss of Δψm, and activation of the mitochondrial caspase cascade, but also cytoprotective autophagy resulting from suppression of the Akt-mTOR pathway. Furthermore, pharmacologic inhibition of the autophagy pathway augments the activation of Bak and resultant mitochondrial damage-mediated apoptosis in Jurkat T cells.
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참고문헌 (23)
참고문헌 신청
Amaravadi RK / 2007 / The roles of therapyinduced autophagy and necrosis in cancer treatment / Clin. Cancer Res 13 : 7271 ~ 7279
Bonfoco E / 1995 / Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-Daspartate or nitric oxide/superoxide in corti
Degenhardt K / 2006 / Autophagy promotes tumor cell survival and restricts necrosis, inflammation, and tumorigenesis / Cancer Cell 10 : 51 ~ 64
Desagher S / 2000 / Mitochondria as the central control point of apoptosis / Trends Cell Biol 10 : 369 ~ 377
Dive C / 1992 / Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry / Biochim. Biophys. Acta 1133 : 275 ~ 285
Bonfoco E / 1995 / Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-Daspartate or nitric oxide/superoxide in corti
Degenhardt K / 2006 / Autophagy promotes tumor cell survival and restricts necrosis, inflammation, and tumorigenesis / Cancer Cell 10 : 51 ~ 64
Desagher S / 2000 / Mitochondria as the central control point of apoptosis / Trends Cell Biol 10 : 369 ~ 377
Dive C / 1992 / Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry / Biochim. Biophys. Acta 1133 : 275 ~ 285
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