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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Watermelon (Citrullus lanatus) is an economically important vegetable crop all over the world, which has functional compounds such as lycopene and citrulline. Gummy stem blight caused by Didymella bryoniae is one of the most devastative diseases in watermelon. Single nucleotide polymorphisms (SNPs), which are genetic variations occurring between individuals with respect to a single base, were often used to construct genetic linkage maps and develop molecular markers linked to a variety of horticultural traits and resistance to several diseases. In this study, we developed high-resolution melting (HRM) markers based on SNPs generated from NGS resequencing of two parents in watermelon. Plant materials were C. lanatus ‘920533’ (female and susceptible parent), C. amarus ‘PI 189225’ (male and resistant parent), and their F1 and F2 progenies. A total of 13.6 Gbp (‘920533’) and 13.1 Gbp (‘PI 189225’) of genomic sequences were obtained using NGS analysis. A total of 6.09 million SNPs between ‘920533’ and ‘PI 189225’ were detected, and 354,860 SNPs were identified as potential HRM primer sets. From these, a total of 330 primer sets for HRM analysis were designed. As a result, a total of 61 HRM markers that have polymorphic melting curves were developed. These HRM markers can be used for the construction of SNP-based linkage maps and for the analysis of quantitative trait loci (QTLs) related to gummy stem blight resistance.
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참고문헌 (32)
참고문헌 신청
Branham SE / 2017 / A GBS-SNP-based linkage map and quantitative trait loci (QTL) associated with resistance to Fusarium oxysporum f. sp. niveum race 2 identified in Citrullus lanatus var. citroides / Theor Appl Genet 130:319~330
dos Santos GR / 2016 / Control of gummy stem blight in watermelon through different management systems / Biosci J 32:371~377
Doyle JJ / 1990 / Isolation of plant DNA from fresh tissue / Focus 12:13~15
Dudley JW / 1992 / Molecular marker information and selection of parents in corn breeding / Crop Sci 32:310~304
Food and Agriculture Organization of the United Nations / Food and agriculture data
dos Santos GR / 2016 / Control of gummy stem blight in watermelon through different management systems / Biosci J 32:371~377
Doyle JJ / 1990 / Isolation of plant DNA from fresh tissue / Focus 12:13~15
Dudley JW / 1992 / Molecular marker information and selection of parents in corn breeding / Crop Sci 32:310~304
Food and Agriculture Organization of the United Nations / Food and agriculture data
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