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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 69 - 77 (9page)
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초록· 키워드
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Screening microorganisms that can produce glucan hydrolases for industrial, environmental, and biomedical applications is important. Herein, we describe a novel approach to perform glucan hydrolase screening— based on analysis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) spectra—which involves degradation of the oligo- and polysaccharides. As a proof-of-concept study, glucan hydrolases that could break down glucans made of several glucose units were used to demonstrate the MALDI-MS-based enzyme assay. First, the enzyme activities of α-amylase and cellulase on a mixture of glucan oligosaccharides were successfully discriminated, where changes of the MALDI-MS profiles directly reflected the glucan hydrolase activities. Next, we validated that this MALDI-MS-based enzyme assay could be applied to glucan polysaccharides (i.e., pullulan, lichenan, and schizophyllan). Finally, the bacterial glucan hydrolase activities were screened on 96-well plate-based platforms, using cell lysates or samples of secreted enzyme. Our results demonstrated that the MALDI-MS-based enzyme assay system would be useful for investigating bacterial glucoside hydrolases in a high-throughput manner.
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참고문헌 (42)
참고문헌 신청
오종민 / 2018 / Characterization of Three Extracellular β-Glucosidases Produced by a Fungal Isolate Aspergillus sp. YDJ14 and Their Hydrolyzing Activity for a Flavone Glycoside / Journal of Microbiology and Biotechnology 28(5):757~764
Fliegmann J / 2004 / An ancient enzyme domain hidden in the putative β-glucan elicitor receptor of soybean may play an active part in the perception of pathogen-associated molecular patterns during broad host resistance / J. Biol. Chem 279:1132~1140
Harris PV / 2010 / Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family / Biochemistry 49:3305~3316
Allgaier M / 2010 / Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community / PLos One 5:e8812
Turner C / 2006 / Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste / Green Chem 8:949~959
Fliegmann J / 2004 / An ancient enzyme domain hidden in the putative β-glucan elicitor receptor of soybean may play an active part in the perception of pathogen-associated molecular patterns during broad host resistance / J. Biol. Chem 279:1132~1140
Harris PV / 2010 / Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family / Biochemistry 49:3305~3316
Allgaier M / 2010 / Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community / PLos One 5:e8812
Turner C / 2006 / Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste / Green Chem 8:949~959
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