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논문 기본 정보

자료유형
학술저널
저자정보
김수환 (차메디텍) 서진형 (충남대학교) 이성진 (차메디텍) 이선영 (충남대학교) 박천웅 (충북대학교)
저널정보
한국생활과학회 한국생활과학회지 한국생활과학회지 제28권 제6호
발행연도
2019.12
수록면
727 - 737 (11page)
DOI
10.5934/kjhe.2019.28.6.727

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초록· 키워드

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The purpose of this study was to examine the anti-oxidative activities of water-ethanol fractions (SP 0, SP 30, SP 60, SP 80, SP 100, respectively) obtained from 70% ethanol extract of Spiraea prunifolia var. Simpliciflora (SP). To determine antioxidant activities of each fraction, total polyphenol content, DPPH and ABTS radicals scavenging activities, and ferric reducing antioxidant power (FRAP) value were measured. Next, we established the inhibitory effects of SP fractions on H2O2-induced oxidative stress in B16F10 cells. The total polyphenol contents of the SP fractions were significantly higher in SP 30, 60, and 80, respectively, than in SP 0 and 100, and DPPH or ABTS radicals scavenging activities and FRAP values of SP 30, 60, and 80, respectively, were higher than those of SP 0. In particular, the total polyphenol content of the SP 60 was 304.77±0.31 mgGAE/g and DPPH radical scavenging activity was 0.03± 0.00 mg/mL, similar to that of the ascorbic acid, and the ABTS radical scavenging activity was at a level of 1/4-1/5 of the ascorbic acid. The FRAP value was 1269.88 ± 12.01 mg ISHE/g. As a result, the SP 60 showed a significantly higher antioxidant capacity compared to other fractions. All of the fractions showed no cytotoxicity up to 125 μg/mL in the B16F10 cells. The SP fractions showed strong cytoprotective effects in H2O2-treated B16F10 cells by reducing the ROS production and DNA damage (p<0.05) and increasing the enzyme activities of SOD and catalase (p<0.05). The anti-oxidant activities were highest at SP 60, followed by SP 80 and SP 30, respectively. Aside from the above results, SP is expected to be used as a functional food and cosmetic material with excellent antioxidant capacity.

목차

Abstract
Ⅰ. 서론
Ⅱ. 연구재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 요약 및 결론
REFERENCE

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