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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 243 - 247 (5page)
- DOI
- 10.7841/ksbbj.2019.34.4.243
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초록· 키워드
오류제보하기
The third-generation gene editing technology, CRISPR-Cas9 system, is derived from the bacterial immune system. These CRISPR-Cas9 systems have recently been used to mutate yeast gene or replace cleavage sites with other DNA. In this study, CRISPR-Cas9 system was applied to delete PHO13 gene of Kluyveromyces marxianus. As a result, only one strain of three transformants, that the CRISPR-Cas9 system was applied, was confirmed partial deletion of PHO13 gene. Sequencing of the PHO13 gene revealed the deletion of cytosine at position 457, which resulted in premature termination of translation as compared to that from the parental strain. The ΔPHO13 strain, K. marxianus 17694-DH2, showed 18.29% and 21.28% improvement in xylose consumption and ethanol production from xylose, respectively, as compared to those form the parental strain.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (25)
참고문헌 신청
Anderson, P. / 1986 / High-efficiency carbohydrate fermentation to ethanol at temperatures above 40 C by Kluyveromyces marxianus var. marxianus isolated from sugar mills / Appl. Environ. Microbiol 51:1314~1320
Hughes, D. / 1984 / The effect of temperature on the kinetics of ethanol production by a thermotolerant strain of Kluveromyces marxianus / Biotechnology letters 6:1~6
Nambu-Nishida, Y. / 2017 / Development of a comprehensive set of tools for genome engineering in a cold-and thermo-tolerant Kluyveromyces marxianus yeast strain / Scientific reports 7:8993
Hoshida, H. / 2014 / Non‐homologous end joining‐mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus / Yeast 31:29~46
Ribeiro, O. / 2007 / Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus / Journal of biotechnology 131:20~26
Hughes, D. / 1984 / The effect of temperature on the kinetics of ethanol production by a thermotolerant strain of Kluveromyces marxianus / Biotechnology letters 6:1~6
Nambu-Nishida, Y. / 2017 / Development of a comprehensive set of tools for genome engineering in a cold-and thermo-tolerant Kluyveromyces marxianus yeast strain / Scientific reports 7:8993
Hoshida, H. / 2014 / Non‐homologous end joining‐mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus / Yeast 31:29~46
Ribeiro, O. / 2007 / Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus / Journal of biotechnology 131:20~26
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