Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures

Kim, Seh Hyun; Lee, Woo Soon; Lee, Na Mi; Chae, Soo Ahn; Yun, Sin Weon

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자료유형: 학술저널

저자정보: Kim, Seh Hyun (Department of Pediatrics, Chung-Ang University College of Medicine) Lee, Woo Soon (Department of Pediatrics, Chung-Ang University College of Medicine) Lee, Na Mi (Department of Pediatrics, Chung-Ang University College of Medicine) Chae, Soo Ahn (Department of Pediatrics, Chung-Ang University College of Medicine) Yun, Sin Weon (Department of Pediatrics, Chung-Ang University College of Medicine)

저널정보: 대한소아청소년과학회 Clinical and Experimental Pediatrics Korean journal of pediatrics 제58권 제4호

발행연도: 2015.1

수록면: 142 - 147 (6page)

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Purpose: The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain. Methods: We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis. Results: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: $5.59%{\pm}2.99%$ vs. $4.80%{\pm}1.37%$, P=0.900; DG: $33.88%{\pm}12.53%$ vs. $15.98%{\pm}2.37%$, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 ($24.51%{\pm}6.05%$ vs. $18.31%{\pm}3.28%$, P=0.373) or DG ($15.72%{\pm}3.47%$ vs. $9.91%{\pm}2.11%$, P=0.134), but was significant in the CA1 ($50.91%{\pm}5.90%$ vs. $32.30%{\pm}3.34%$, P=0.004). Conclusion: Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.

#Hippocampus #Hypoxia #Brain

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