Comparison of Two Methods to Extract DNA from Formalin-Fixed, Paraffin-Embedded Tissues and their Impact on EGFR Mutation Detection in Non-small Cell Lung Carcinoma

Hu, Yu-Chang; Zhang, Qian; Huang, Yan-Hua; Liu, Yu-Fei; Chen, Hong-Lei

추천

검색

자료유형: 학술저널

저자정보: Hu, Yu-Chang (Department of Pathology, The First College of Clinical Medical Sciences, China Three Gorges University) Zhang, Qian (Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan University) Huang, Yan-Hua (Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan University) Liu, Yu-Fei (Department of Pathology, The First College of Clinical Medical Sciences, China Three Gorges University) Chen, Hong-Lei (Department of Pathology, School of Basic Medical Science, Wuhan University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제6호

발행연도: 2014.1

수록면: 2,733 - 2,737 (5page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Objective: Molecular pathology tests are often carried for clinicopathological diagnosis and pathologists have established large collections of formalin-fixed, paraffin-embedded tissue (FFPE) banks. However, extraction of DNA from FFPE is a laborious and challenging for researchers in clinical laboratories. The aim of this study was to compare two widely used DNA extraction methods: using a QIAamp DNA FFPE kit from Qiagen and a Cobas Sample Preparation Kit from Roche, and evaluated the effect of the DNA quality on molecular diagnostics. Methods: DNA from FFPE non-small cell lung carcinoma tissues including biopsy and surgical specimens was extracted with both QIAamp DNA FFPE and Cobas Sample Preparation Kits and EGFR mutations of non-small cell lung carcinomas were detected by real-time quantitative PCR using the extracted DNA. Results and Conclusion: Our results showed that DNA extracted by QIAamp and Cobas methods were both suitable to detect downstream EGFR mutation in surgical specimens. Howover, Cobas method could yield more DNA from biopsy specimens, and gain much better EGFR mutation results.

#DNA extraction #cobas #QIAamp #EGFR mutation

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

이 논문의 저자 정보

Hu, Yu-Chang

소속기관 Department of Pathology, The First College of Clinical Medical Sciences, China Three Gorges Universi