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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 219 - 219 (1page)
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Methane has powerful potential as next-generation feedstock because it was buried in the status of shale gas over the world and cheaper than other feedstock like sugar. Methanotroph can utilize methane as sole carbon source and produce value-added materials such as succinic acid.
In this study, Methylomonas sp. DH-1, type I obligate methanotroph, was used to produce succinic acid. Succinate dehydrogenase gene was replaced with glyoxylate shunt genes (isocitrate lyase and malate synthase) using the cre-lox system integrated in genome of wild type strain. To improve microbial activities, adaptive laboratory evolution (ALE) was performed with the mutant strain. The new strain (CA40) showing improved methane consumption rate and faster growth than the parental strain was obtained by transferring cells 40 times. The maximum methane consumpt ... 전체 초록 보기
In this study, Methylomonas sp. DH-1, type I obligate methanotroph, was used to produce succinic acid. Succinate dehydrogenase gene was replaced with glyoxylate shunt genes (isocitrate lyase and malate synthase) using the cre-lox system integrated in genome of wild type strain. To improve microbial activities, adaptive laboratory evolution (ALE) was performed with the mutant strain. The new strain (CA40) showing improved methane consumption rate and faster growth than the parental strain was obtained by transferring cells 40 times. The maximum methane consumpt ... 전체 초록 보기
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