Study on the bio-function of lipA gene in Aspergillus flavus

Wenzhao Bai; Tiejun Feng; Faxiu Lan; Guanglan Lin; Guanglan Lin; Opemipo Esther Fasoyin; Yaju Liu; Kunzhi Jia

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자료유형: 학술저널

저자정보: Wenzhao Bai (Shaanxi University of Technology) Tiejun Feng (Fujian Agriculture and Forestry University) Faxiu Lan (Fujian Agriculture and Forestry University) Guanglan Lin (Fujian Agriculture and Forestry University) Guanglan Lin (Fujian Agriculture and Forestry University) Opemipo Esther Fasoyin (Fujian Agriculture and Forestry University) Yaju Liu (Fujian Agriculture and Forestry University) Kunzhi Jia (Fujian Agriculture and Forestry University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.41 No.1

발행연도: 2019.1

수록면: 107 - 111 (5page)

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Lipoic acid synthase (LipA) plays a role in lipoic acid synthesis and potentially affects the levels of acetyl-CoA, the critical precursor of tricarboxylic acid (TCA) cycle. Considering the potential effect of LipA on TCA cycle, whether the enzyme is involved in the growth and aflatoxin B1 (AFB1) biosynthesis, the significant events in Aspergillus flavus is yet known. The study was designed to explore the role of lipA gene in A. flavus, including growth rate, conidiation, sclerotia formation, and biosynthesis of AFB1. LipA coding lipoic acid synthetase was knocked out using homologous recombination. The role of lipA gene in A. flavus morphogenesis (including colony size, conidiation, and sclerotia formation) was explored on various media, and the bio-function of lipA gene in the biosynthesis of AFB1 was analyzed by thin layer chromatography analysis. The growth was suppressed in △lipA. The formation of conidia and sclerotia was also reduced when lipA gene was deleted. Moreover, AFB1 was down-regulated in ΔlipA compared with WT controls. LipA plays a role in the development of A. flavus and AFB1 biosynthesis, contributing to the full understanding of the lipA bio-function in A. flavus.

#Aspergillus flavus · Lipoic acid synthetase · lipA · Aflatoxin B1

참고문헌 (16)

참고문헌 신청

Bennett JW / 2003 / Mycotoxins / Clin Microbiol Rev 16:497~516

Chang PK / 2010 / Development and refinement of a high-efficiency gene-targeting system for Aspergillus flavus / J Microbiol Methods 81(3):240~246

Cronan JE / 2014 / Biotin and lipoic acid: synthesis, attachment, and regulation, / EcoSal Plus 3

Frey PA / 2008 / The radical SAM superfamily / Crit Rev Biochem Mol Biol 43(1):63~88

Herbert AA / 1968 / Biochemical and genetic studies with lysine + methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants / J Gen Microbiol 53:363~381

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Wenzhao Bai

소속기관 Shaanxi University of Technology

주요연구분야 자연과학 > 생물학

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Tiejun Feng