지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 15 - 21 (7page)
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초록· 키워드
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The in vitro alternative assay for evaluating skin sensitizing potentials (SSP) called human Cell Line Activation Test (h-CLAT) has been recently adopted as an international test guideline. The present study was proceeded to pursue the domestic establishment and expansion of h-CLAT. The standard operational procedure was prepared in detail by the lead laboratory (LL) and transferred to one participating laboratory (PL). The LL demonstrated technical proficiency for the 10 substances listed in OECD TG 442E. The PL also demonstrated a certain level of proficiency, in that 4-phenylenediamine and 4-aminobenzoic acid distributed with coded names were correctly classified as positive and negative on SSP, respectively, and other indices including 75% cell viability (CV75) and relative fluorescence intensity (RFI) % for CD54 and CD86 were fell within the reference range. Methyl methacrylate, nickel chloride, and resorcinol, which are inconsistent in predicting SSP through various alternative test methods, were unanimously predicted as negative, positive, and positive on SSP by the two laboratories for the first time through h-CLAT. Both chlorobenzene and sodium lauryl sulfate with the existing h-CLAT report as positive and negative, respectively, were predicted as negative by the PL, which could contribute toward overall categorization into non-skin sensitizer.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
식품의약품안전평가원 / 2017 / 화장품 피부감작성 동물대체시험법(인체 세포주 활성화 방법, h-CLAT) 가이드라인(민원인 안내서)
Ahn IY / 2016 / Performance standard-based validation study for local lymph node assay: 5-bromo-2-deoxyuridineflow cytometry method / Regulatory Toxicology and Pharmacology 80:183~194
Ashikaga T / 2006 / Development of an in vitro skin sensitization test using human cell lines : The human Cell Line Activation Test(h-CLAT)I / Optimization of the h-CLAT protocol. Toxicology In Vitro 20:767~773
Casati S. / 2018 / Integrated approaches to testing and assessment / Basic & Clinical Pharmacology & Toxicology 123:51~55
Clouet E / 2017 / Comparison and validation of an in vitro skin sensitization strategy using a data set of 33 chemical references / Toxicology in Vitro 45:374~385
Ahn IY / 2016 / Performance standard-based validation study for local lymph node assay: 5-bromo-2-deoxyuridineflow cytometry method / Regulatory Toxicology and Pharmacology 80:183~194
Ashikaga T / 2006 / Development of an in vitro skin sensitization test using human cell lines : The human Cell Line Activation Test(h-CLAT)I / Optimization of the h-CLAT protocol. Toxicology In Vitro 20:767~773
Casati S. / 2018 / Integrated approaches to testing and assessment / Basic & Clinical Pharmacology & Toxicology 123:51~55
Clouet E / 2017 / Comparison and validation of an in vitro skin sensitization strategy using a data set of 33 chemical references / Toxicology in Vitro 45:374~385
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