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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.
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참고문헌 (48)
참고문헌 신청
Bai C / 1996 / Cleavage of RNA hairpins mediated by a developmentally regulated CCCH zinc finger protein / Mol. Cell. Biol 16:6661~6667
Bhuiyan MSU / 2011 / Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea / Plant Cell Tiss. Organ Cult 105:85~91
Carballo E / 1998 / Feedback inhibition of macrophage tumor necrosis factor-α production by tristetraprolin / Science 281:1001~1005
Chen M / 2007 / GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants / Biochem. Biophys. Res. Commun 353:299~305
Fan L / 1997 / Antisense suppression of phospholipase D alpha retards abscisic acid- and ethylenepromoted senescence of postharvest Arabidopsis leaves / Plant Cell 9:2183~2196
Bhuiyan MSU / 2011 / Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea / Plant Cell Tiss. Organ Cult 105:85~91
Carballo E / 1998 / Feedback inhibition of macrophage tumor necrosis factor-α production by tristetraprolin / Science 281:1001~1005
Chen M / 2007 / GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants / Biochem. Biophys. Res. Commun 353:299~305
Fan L / 1997 / Antisense suppression of phospholipase D alpha retards abscisic acid- and ethylenepromoted senescence of postharvest Arabidopsis leaves / Plant Cell 9:2183~2196
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