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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한예방치과학회 International Journal of Clinical Preventive Dentistry International Journal of Clinical Preventive Dentistry Vol.14 No.3
- 발행연도
- 2018.1
- 수록면
- 197 - 202 (6page)
이용수
초록· 키워드
오류제보하기
Objective: Gingival inflammation is one of the main causes that can be associated with a lot of periodontal diseases. Among various periodontal pathogens, Porphyromonas gingivalis can be recognized as one of the main causes in the progression of the periodontal inflammation. In this study, the anti-inflammatory activity of Gelidium amansii ethanol extract (GAEE) and its molecular mechanism was investigated in P. gingivalis lipopolysaccharide (LPS-PG) stimulated human gingival fibroblast (HGF)-1 cells. Methods: The concentration of nitric oxide (NO) and prostaglandin E2 (PGE2) production was estimated by biochemical analysis. Protein expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and their upstream signaling molecules were analyzed by Western blot analysis. Results: LPS-PG induced NO and PGE2 production as well as their corresponding enzymes, iNOS and COX-2, were significantly attenuated by GAEE treatment without cytotoxicity. The molecular mechanism was also investigated to determine whether this response was related to the inflammatory transcription factors, nuclear factor (NF)-B and activator protein (AP)-1, or their upstream signaling molecules, mitogen-activated protein kinases (MAPKs) and phosphoinositide 3-kinase (PI3K)/Akt. Phosphorylated status of p65 and c-jun, each subunit of NF-B and AP-1, was dose-dependently inhibited by GAEE treatment. In addition, GAEE treatment inhibited phosphorylation of extracellular regulated kinase (ERK) but did not give any effect on other MAPKs and PI3K/Akt signaling molecules. Conclusion: Consequently, GAEE ameliorates LPS-PG-induced inflammatory responses by blocking NF-B, AP-1 and ERK activation in HGF-1 cells. Therefore, GAEE may be utilized as a potential anti-inflammatory agent by modulating NO and PGE2 production in the periodontium.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Lawrence T / 2002 / Anti-inflammatory lipid mediators and insights into the resolution of inflammation / Nat Rev Immunol 2:787~795
How KY / 2016 / Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line / Front Microbiol 7:53
Milward MR / 2003 / Classification of periodontal diseases: where were we? Where are we now? Where are we going? / Dent Update 30:37~44
Williams RO / 1992 / Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis / Proc Natl Acad Sci USA 89:9784~9788
Hajishengallis G / 2014 / Breaking bad: manipulation of the host response by Porphyromonas gingivalis / Eur J Immunol 44:328~338
How KY / 2016 / Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line / Front Microbiol 7:53
Milward MR / 2003 / Classification of periodontal diseases: where were we? Where are we now? Where are we going? / Dent Update 30:37~44
Williams RO / 1992 / Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis / Proc Natl Acad Sci USA 89:9784~9788
Hajishengallis G / 2014 / Breaking bad: manipulation of the host response by Porphyromonas gingivalis / Eur J Immunol 44:328~338
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