Implantation of Bone Marrow Stromal Cell Sheets Derived from Old Donors Supports Bone Tissue Formation

Manabu Akahane; Takamasa Shimizu; Yusuke Inagaki; Tsutomu Kira; Takuya Egawa; Akinori Okuda; Tadanobu Onishi; Tomoaki Imamura; Yasuhito Tanaka

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자료유형: 학술저널

저자정보: Manabu Akahane (Nara Medical University) Takamasa Shimizu (Nara Medical University) Yusuke Inagaki (Nara Medical University) Tsutomu Kira (Nara Medical University) Takuya Egawa (Nara Medical University) Akinori Okuda (Nara Medical University) Tadanobu Onishi (Nara Medical University) Tomoaki Imamura (Nara Medical University) Yasuhito Tanaka (Nara Medical University)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제15권 제1호

발행연도: 2018.1

수록면: 89 - 100 (12page)

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The purpose of this study was to evaluate the osteogenesis ability of osteogenic matrix cell sheets (OMCS) derived from old donor cells. Bone marrow stromal cells (BMSC) were obtained from young (7-week-old) and old (1-yearold) Fischer344 rats donors and cultured with modified Eagle’s medium (MEM group) alone or containing dexamethasone (Dex; 10 nM) and ascorbic acid phosphate (AscP; 0.28 mM) (Dex/AscP group). We prepared four in vitro experimental groups: (1) young MEM, (2) young Dex/AscP, (3) old MEM and (4) old Dex/AscP. Cell proliferation and osteogenic marker mRNA expression levels were evaluated in vitro. To assess bone formation in vivo, the cells of each group were combined with beta tricalcium phosphate (TCP) disks followed by implantation in recipient rats. The in vitro study showed significant differences in the mRNA expression of osteocalcin, ALP, and BMP2 between MEM and Dex/AscP groups. Bone formation following implantation was observed upon histological analyses of all groups. TCP combined with OMCS (OMCS/TCP group) resulted in enhanced bone formation compared to that following combination with BMSC (BMSC/ TCP). The osteocalcin content of the OMCS/TCP group 4 weeks after implantation was significantly higher than that in the BMSC/TCP construct for both young and old donors. The present study clearly indicated that OMCS could be generated from BMSCs of old as well as young donors using a mechanical retrieval method. Thus, through its usage of OMCS, this method may represent a potentially effective therapeutic option for cell-based therapy in elderly patients.

참고문헌 (36)

참고문헌 신청

Kawate K / 2006 / Tissue-engineered approach for the treatment of steroid-induced osteonecrosis of the femoral head: transplantation of autologous mesenchymal stem cells cultured with beta-tricalcium phosphate ceramics and free vascularized fibula / Artif Organs 30:960~962

Zhao X / 2016 / Chondrogenesis by bone marrow-derived mesenchymal stem cells grown in chondrocyte-conditioned medium for auricular reconstruction / J Tissue Eng Regen Med.

Ohgushi H / 1999 / Stem cell technology and bioceramics: from cell to gene engineering / J Biomed Mater Res 48:913~927

Brazelton TR / 2000 / From marrow to brain: expression of neuronal phenotypes in adult mice / Science 290:1775~1779

Squillaro T / 2016 / Clinical trials with mesenchymal stem cells: an update / Cell Transplant 25:829~848

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