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논문 기본 정보

자료유형
학술저널
저자정보
Jueun Jang (Kyungpook National University) In Jung Kim (Kyungpook National University) Suhyeung Kim (Kyungpook National University) Jamin Shin (Kyungpook National University) Sejin Geum (Kyungpook National University) Soo Rin Kim (Kyungpook National University)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.37 No.2(Wn.181)
발행연도
2022.6
수록면
49 - 57 (9page)

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Sargassum horneri is a sea-drifting brown macroalga often found along the coast of East Asian countries. It was recently found to be drifting from China toward Jeju Island in South Korea, causing damage to fisheries and vessels. Being considered as a marine waste, a huge amount of S. horneri was collected in the past 5 years, but an efficient and proper way to treat it has still not been found. Therefore, it is required to develop technologies to tackle this issue. Here, we conducted bioconversion of S. horneri by the yeast Saccharomyces cerevisiae in order to utilize it as a biomass source for producing ethanol. First, S. cerevisiae was engineered to extend its substrate range to mannitol, which is one of the major components of brown algae. Activation of the native HXT17 and MAN2 genes enabled the yeast to metabolize mannitol as the sole carbon source. Impact of pretreatment conditions, the type of hydrolytic enzymes, and biomass solid loadings on the ethanol production by the yeast were evaluated. The highest ethanol productivity was obtained when the biomass was pretreated at 121oC and ethanol concentration was the highest when the biomass loading was 24% (w/v), giving the maximum concentrations of monosaccharides and ethanol of 47.29 g/L and 22.94 g/L, respectively. The results obtained from this study suggest possible utilization of S. horneri as a raw material for cellulosic bioethanol production.

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Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
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