KIF26B-AS1 Regulates TLR4 and Activates the TLR4 Signaling Pathway to Promote Malignant Progression of Laryngeal Cancer

Li Li; Han Jiahui; Zhang Shujia; Dong Chunguang; Xiao Xiang

doi:10.4014/jmb.2203.03037

추천

검색

자료유형: 학술저널

저자정보: Li Li (Department of Otolaryngology Head and Neck Surgery The First People’s Hospital of Lianyungang City) Han Jiahui (Department of Otolaryngology Head and Neck Surgery The First People’s Hospital of Lianyungang City) Zhang Shujia (Department of Otolaryngology Head and Neck Surgery The First People’s Hospital of Lianyungang City) Dong Chunguang (Department of Otolaryngology Head and Neck Surgery The First People’s Hospital of Lianyungang City) Xiao Xiang (Department of Otolaryngology Head and Neck Surgery The First People’s Hospital of Lianyungang City)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제10호

발행연도: 2022.10

수록면: 1,344 - 1,354 (11page)

DOI: 10.4014/jmb.2203.03037

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Laryngeal cancer is one of the highest incidence, most prevalently diagnosed head and neck cancers, making it critically necessary to probe effective targets for laryngeal cancer treatment. Here, realtime quantitative reverse transcription PCR (qRT-PCR) and western blot analysis were used to detect gene expression levels in laryngeal cancer cell lines. Fluorescence in situ hybridization (FISH) and subcellular fractionation assays were used to detect the subcellular location. Functional assays encompassing Cell Counting Kit-8 (CCK-8), 5-ethynyl-2’-deoxyuridine (EdU), transwell and wound healing assays were performed to examine the effects of target genes on cell proliferation and migration in laryngeal cancer. The in vivo effects were proved by animal experiments. RNA-binding protein immunoprecipitation (RIP), RNA pulldown and luciferase reporter assays were used to investigate the underlying regulatory mechanisms. The results showed that KIF26B antisense RNA 1 (KIF26B-AS1) propels cell proliferation and migration in laryngeal cancer and regulates the toll-like receptor 4 (TLR4) signaling pathway. KIF26B-AS1 also recruits FUS to stabilize TLR4 mRNA, consequently activating the TLR4 signaling pathway. Furthermore, KIF26B-AS1 plays an oncogenic role in laryngeal cancer via upregulating TLR4 expression as well as the FUS/TLR4 pathway axis, findings which offer novel insight for targeted therapies in the treatment of laryngeal cancer patients.

#KIF26B-AS1 #FUS #TLR4 #TLR4 signaling pathway #laryngeal cancer

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!