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논문 기본 정보

자료유형
학술저널
저자정보
Kang Jin Yong (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Lee Moeun (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Song Jung Hee (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Choi Eun Ji (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Kim Da un (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Lim Seul Ki (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) Kim Namhee (Research and Development Division World Institute of Kimchi Gwangju 61755 Republic of Korea) 장지윤 (세계김치연구소)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제12호
발행연도
2022.12
수록면
1,583 - 1,588 (6page)
DOI
10.4014/jmb.2209.09026

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초록· 키워드

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In this study, we investigated the effect of lactic acid bacteria (LAB) strains used as starters for kimchi fermentation, namely Lactococcus lactis WiKim0124, Companilactobacillus allii WiKim39, Leuconostoc mesenteroides WiKim0121Leuconostoc mesenteroides WiKim33, and Leuconostoc mesenteroides WiKim32, on the intestinal epithelial tight junctions (TJs). These LAB strains were not cytotoxic to Caco-2 cells at 500 μg/ml concentration. In addition, hydrogen peroxide (H2O2) decreased Caco-2 viability, but the LAB strains protected the cells against H2O2-induced cytotoxicity. We also found that lipopolysaccharide (LPS) promoted Caco-2 proliferation; however, no specific changes were observed upon treatment with LAB strains and LPS. Our evaluation of the permeability in the Caco-2 monolayer model confirmed its increase by both LPS and H2O2. The LAB strains inhibited the increase in permeability by protecting TJs, which we evaluated by measuring TJ proteins such as zonula occludens-1 and occludin, and analyzing them by western blotting and immunofluorescence staining. Our findings show that LAB strains used for kimchi fermentation can suppress the increase in intestinal permeability due to LPS and H2O2 by protecting TJs. Therefore, these results suggest the possibility of enhancing the functionality of kimchi through its fermentation using functional LAB strains.

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