지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.2
- 수록면
- 187 - 196 (10page)
- DOI
- 10.1007/s13258-021-01177-x
이용수
초록· 키워드
오류제보하기
Background
Madin–Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered seriously because of high tumorigenicity. Micro RNAs (miRNAs) that are involved in the tumorigenicity of MDCK cells have been never been reported.
Objective
To reveal the role of miRNA in the tumorigenic phenotype of MDCK cell line.
Methods
The miRNA expression profiles of two monoclonal MDCK cells (M09CL and M35CL) with low tumorigenicity and one MDCK cell line (M73P) with high tumorigenicity were characterized and investigated by using small RNA-seq technology.
Results
A total of 5 known miRNAs and 5 novel miRNAs were highly expressed in M73P. In addition, 4 known miRNAs and 4 novel miRNAs were highly expressed in M09CL and M35CL. The target genes of the differentially expressed miRNAs were significantly enriched in several biological processes, and the majority of these genes were involved in pathways in cancer and the MAPK signaling pathway. Through interaction analysis, 4 up-regulated miRNAs (cfa-miR-452, cfa-miR-8826, cfa-miR-224, and cfa-miR-2387) and their crucial target genes related to the tumor regulation network were identified. Results indicated these 4 miRNAs might play crucial roles in the tumorigenesis of MDCK cells.
Conclusion
Our findings, which were based on the functional prediction of miRNAs and target genes, suggested that miRNAs might influence the tumorigenicity of MDCK cells by regulating target genes. Moreover, the results provided important data for understanding the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Genetic features of Haliotis discus hannai by infection of vibrio and virus
Genes & Genomics
2020 .01
-
Determinants of Functional MicroRNA Targeting
Molecules and Cells
2023 .01
-
Genome-wide identification of drought-responsive microRNAs and their target genes in Chinese jujube by deep sequencing
Genes & Genomics
2023 .02
-
Identification and comparative analysis of the pearl oyster Pinctada fucata hemocytes microRNAs in response to Vibrio alginolyticus infection
Genes & Genomics
2017 .01
-
In Silico Study of miRNA Based Gene Regulation, Involved in Solid Cancer, by the Assistance of Argonaute Protein
Genomics & informatics
2016 .01
-
Genomic identification of microRNA promoters and their cis-acting elements in Populus
Genes & Genomics
2016 .01
-
Disease-miRNAdb: a manually-curated database for the investigation of the microRNA-human disease relationship
Genes & Genomics
2017 .01
-
Deep sequencing reveals microRNA signature is altered in the rat epididymis following bilateral castration
Genes & Genomics
2019 .01
-
Characterization and differential expression of microRNA in skeletal muscle of Laiwu and Yorkshire pig breeds
Genes & Genomics
2017 .01
-
Identification of MicroRNAs as potential biomarkers for detecting ischemic stroke
Genes & Genomics
2022 .01
-
Identification of differentially expressed miRNAs after Lactobacillus reuteri treatment in the ileum mucosa of piglets
Genes & Genomics
2020 .01
-
Discovery and profiling of microRNAs and their targets in Paulownia ‘Yuza 1’ plants via high-throughput sequencing and degradome analysis
Genes & Genomics
2016 .01
-
MicroRNA Target Recognition: Insights from Transcriptome-Wide Non-Canonical Interactions
Molecules and Cells
2016 .05
-
Generation of a High-Growth Influenza Vaccine Strain in MDCK Cells for Vaccine Preparedness
Journal of Microbiology and Biotechnology
2018 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
Effective Fluorescent and colorimetric sensor for miRNA
한국진공학회 학술발표회초록집
2019 .08
-
Comparative study of predicted miRNA between Indonesia and China (Wuhan) SARS-CoV-2: a bioinformatics analysis
Genes & Genomics
2021 .09
-
Physiological responses and small RNAs changes in maize under nitrogen deficiency and resupply
Genes & Genomics
2019 .01
-
High throughput sequencing of herbaceous peony small RNAs to screen thermo-tolerance related microRNAs
Genes & Genomics
2017 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0