지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
meso-Diaminopimelic acid decarboxylase from Corynebacterium glutamicum (CgDAPDC) is the key enzyme for theproduction of L-lysine and it catalyzes meso-DAP to produce the final product, L-lysine. The CgDAPDC was overexpressedand purified to homogeneity by Ni-NTA affinity and size-exclusion chromatography. The CgDAPDC protein was crystallizedusing sitting-drop vapor-diffusion method in the presence of 0.8 M sodium citrate tribasic and 0.1 M sodium cacodylate,pH 6.5 at 293 K. X-ray diffraction data were collected to a maximum resolution of 2.4 A. The crystal belonged to spacegroup P21212, with unit cell parameters a = 114.54 A, b = 91.702 A, c = 95.161, α = β = γ = 90°. With one molecules perasymmetric unit, the crystal volume per unit protein mass was 2.64 A3 Da-1, which correspond to a solvent content ofapproximately 53.37%.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Becker, J / 2012 / Bio-based production of chemicals, materials and fuels-Corynebacterium glutamicum as versatile cell factory / Curr Opin Biotechnol 23:631~640
Blombach, B / 2011 / Corynebacterium glutamicum tailored for efficient isobutanol production / Appl Environ Microbiol 77:3300~3310
Buschke, N / 2011 / Metabolic engineering of Corynebacterium glutamicum for production of 1, 5-diaminopentane from hemicellulose / Biotechnology Journal 6:306~317
Chen, Z / 2016 / Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose / Metabolic Engineering 33:12~18
Emsley, P / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Blombach, B / 2011 / Corynebacterium glutamicum tailored for efficient isobutanol production / Appl Environ Microbiol 77:3300~3310
Buschke, N / 2011 / Metabolic engineering of Corynebacterium glutamicum for production of 1, 5-diaminopentane from hemicellulose / Biotechnology Journal 6:306~317
Chen, Z / 2016 / Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose / Metabolic Engineering 33:12~18
Emsley, P / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
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