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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 115 - 125 (11page)
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RNA interference (RNAi) occurs naturally in plants and is involved in developmental gene regulation as well as in transposon silencing and defence against viruses.
RNAi is initiated by small double-stranded RNAs (dsRNAs) of different origins, eventually leading to the silencing of genes with complementary sequences. Besides its use as a basic tool to study gene function, RNAi has widely been exploited for the generation of pathogen resistant crops and for modifying plant metabolism. For the elucidation of gene functions, silencing-inducing dsRNA is often delivered transiently by means of agroinfiltration.
This method is independent of plant regeneration and allows rapid testing of multiple silencing constructs. To get more insight into the applicability of transient gene silencing, we compared a variety of different transient and stable approaches to induce silencing of the b-glucuronidase (gus) transgene by the expression of gus hairpin (hpGus) transcripts. While stable expression of the hairpin always resulted in specific gene silencing, transiently expressed hpGus transcripts could only trigger silencing when the hairpin construct was introduced simultaneously with or prior to the target gene.
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등록된 정보가 없습니다.
참고문헌 (52)
참고문헌 신청
Andrieu A / 2012 / An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves / Rice (N Y) 5:23
Bernstein E / 2001 / Role for a bidentate ribonuclease in the initiation step of RNA interference / Nature 409:363~366
Bhaskar PB / 2009 / Agrobacterium-mediated transient gene expression and silencing:a rapid tool for functional gene assay in potato / PLoS One 4:e5812
Bradford MM / 1976 / A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding / Anal Biochem 72:248~254
Braunstein TH / 2002 / Specific degradation of 30 regions of GUS mRNA in posttranscriptionally silenced tobacco lines may be related to 50–30spreading of silencing / RNA 8:1034~1044
Bernstein E / 2001 / Role for a bidentate ribonuclease in the initiation step of RNA interference / Nature 409:363~366
Bhaskar PB / 2009 / Agrobacterium-mediated transient gene expression and silencing:a rapid tool for functional gene assay in potato / PLoS One 4:e5812
Bradford MM / 1976 / A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding / Anal Biochem 72:248~254
Braunstein TH / 2002 / Specific degradation of 30 regions of GUS mRNA in posttranscriptionally silenced tobacco lines may be related to 50–30spreading of silencing / RNA 8:1034~1044
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