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논문 기본 정보

자료유형
학술저널
저자정보
Tursun Ayse Ozlem (University of Malatya Turgut Ozal) Sipahioglu Hikmet Murat (University of Malatya Turgut Ozal) Telci Isa (University of Applied Sciences Dogu Campus)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제15권 제5호
발행연도
2021.10
수록면
663 - 672 (10page)
DOI
10.1007/s11816-021-00712-2

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Sage (Salvia officinalis L.) is not found in Turkey's natural flora; however, its cultivation has been increasing in recent years. The species is widely cultivated in many provinces of the Aegean and the Mediterranean regions. There has been no informa- tion regarding genetic characteristics and the intra-individual phylogeny of cultivated sage. The levels of genetic relatedness of 19 Salvia samples from Izmir were determined by molecular tools. The sequences from the internal transcriber spacer (ITS) region of 18S?28S nuclear ribosomal DNA (nrDNA) locus were amplified by PCR and sequenced. Approximately, a 710?bp single amplified product was obtained in all genotypes. Of the nineteen Salvia specimens, six were identified as Salvia x sylvestris L. and the remaining 13 specimens were S. officinalis. The sequences of both species shared no similar secondary structures. The ITS region of all specimens contains several indels and substitutions. In the phylogenetic dendrogram, three major clusters (I, II, and III) and two independent branches (IV and V) were observed. Ninety-seven percent of local Salvia specimens were clustered in two branches (I and II), indicating a high diversity of genetic relatedness. Sequence divergence was higher among the specimens of S. officinalis than the specimens of Salvia x sylvestris.

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