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논문 기본 정보

자료유형
학술저널
저자정보
주다혜 (호서대학교 한방화장품과학과) 유단희 (호서대학교 한방화장품과학과) 이수연 (호서대학교) 이진영 (호서대학교)
저널정보
한국미용학회 한국미용학회지 한국미용학회지 제21권 제5호
발행연도
2015.10
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888 - 894 (7page)

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The purpose of this study was to research the whitening effects and developing by cosmetics of the water extract from Astragalus membranceus Bunge, which is one of the most popular health-promoting herb in herbal medications. We performed tyrosinase inhibition assay, reverse transcription- polymerase chain reaction (RT-PCR) and western blot for whitening effects. Also we measured MTT assay for cell viability. The results were obtained as follows : For whitening effect, tyrosinase inhibition rate of water extract from Astragalus membranceus Bunge showed more than 44% at 1,000 μg/ml concentration. Cell toxicity effect on melanoma cells (B16F10) of water extract from Astragalus membranceus Bunge showed 81% with toxicity at 50 μg/ml concentration. So we were measured at a concentrations of 5, 10 and 50 μg/ml in all experiments involving cell. In addition, whitening related mRNAs including microphthalmia associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), tyrosinase were reduced by Astragalus membranceus Bunge. We also found Astragalus membranceus Bunge transiently decreased protein kinase A (PKA) which is known to be upstream to the down regulation of MITF and tyrosinase. But phosphorylation of extracellular signal related kinase (pERK) were increased by Astragalus membranceus Bunge. These results imply that Astragalus membranceus Bunge decrease melanogenesis via ERK activation and subsequent down regulation of MITF and tyrosinase. Therefore, in this paper we would like to suggest the potent usage of Astragalus membranceus Bunge as materials of functional cosmetics by confirming whitening activity related with melanin content.

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