Purpose: This study aims to identify the moderating effect of nursing organizational culture on the relationship between resilience and clinical competence among new graduate nurses. Methods: A survey was conducted from March 14 to July 14, 2023, targeting 210 new nurses with 3 to 12 months of experience, working in three general hospitals in Seoul and Gyeonggi-do. Data from 193 respondents were analyzed using descriptive statistics, correlation, and hierarchical regression. Results: The resilience of new graduate nurses was moderate and their clinical competence was rated as good. They perceived a strong hierarchy-oriented culture, followed by a relationship-oriented culture, an innovation-oriented culture, and lastly, a task-oriented culture. Clinical competence was higher with greater resilience (β=.40, p<.001) and stronger perception of hierarchy-oriented culture (β=.16, p=.013). The interaction between resilience and innovation-oriented culture (β=.22, p=.012) was statistically significant, indicating that the effect of resilience on clinical competence was significantly higher when the innovation-oriented culture was strongly perceived. Conclusion: To improve the clinical competence of new graduate nurses, it is essential to enhance their personal resilience and, at an organizational level, to strengthen the positive aspects of hierarchy-oriented and innovation-oriented cultures.