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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 409 - 413 (5page)
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To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both TgMTP1 (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that TgMTP1-overexpressing plants may be useful for phytoremediation of soil.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Baker AJ / 1981 / Accumulators and excluders-strategies in the response of plants to heavy metals / J. Plant Nutrit 3 : 643 ~ 654
Etim EE / 2012 / Phytoremediation and Its Mechanisms / International Journal of Environment and Bioenergy 2 (3) : 120 ~ 136
Gustin JL / 2009 / MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants / Plant J 57 : 1116
Kil EJ / 2015 / Research of phytoremediation possibility using TgMTP1 overexpressing plants / Sungkyunkwan University Journal of Biotechnology and Bioengineering 21 : 1 ~ 5
Kim D / 2004 / The plant CDF family member TgMTP1 from the Ni/Zn hyperaccumulator Thlaspi goesingense acts to enhance efflux of Zn at the plasma membrane when expressed in Sa
Etim EE / 2012 / Phytoremediation and Its Mechanisms / International Journal of Environment and Bioenergy 2 (3) : 120 ~ 136
Gustin JL / 2009 / MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants / Plant J 57 : 1116
Kil EJ / 2015 / Research of phytoremediation possibility using TgMTP1 overexpressing plants / Sungkyunkwan University Journal of Biotechnology and Bioengineering 21 : 1 ~ 5
Kim D / 2004 / The plant CDF family member TgMTP1 from the Ni/Zn hyperaccumulator Thlaspi goesingense acts to enhance efflux of Zn at the plasma membrane when expressed in Sa
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