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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 223 - 228 (6page)
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초록· 키워드
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Objectives The aim of this study was to investigate the expression of 7 different sirtuin genes (SIRT1-SIRT7) in human dental pulp cells (HDPCs), and to determine the role of SIRTs in the odontoblastic differentiation potential of HDPCs.
Materials and Methods HDPCs were isolated from freshly extracted third molar teeth of healthy patients and cultulred in odontoblastic differentiation inducing media. Osteocalcin (OCN) and dentin sialophosphoprotein (DSPP) expression was analyzed to evaluate the odontoblastic differentiation of HDPCs by reverse transcription-polymerase chain reaction (RT-PCR), while alizarin red staining was used for the mineralization assay. To investigate the expression of SIRTs during odontoblastic differentiation of HDPCs, real time PCR was also performed with RT-PCR.
Results During the culture of HDPCs in the differentiation inducing media, OCN, and DSPP mRNA expressions were increased. Mineralized nodule formation was also increased in the 14 days culture. All seven SIRT genes were expressed during the odontogenic induction period. SIRT4 expression was increased in a time-dependent manner.
Conclusions Our study identified the expression of seven different SIRT genes in HDPCs, and revealed that SIRT4 could exert an influence on the odontoblast differentiation process. Further studies are needed to determine the effects of other SIRTs on the odontogenic potential of HDPCs.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Fouad AF / 2011 / Pathways of the pulp / Mosby Elsevier : 504 ~ 528
Guarente L / 2013 / Calorie restriction and sirtuins revisited / Genes Dev 27 : 2072 ~ 2085
Haigis MC / 2010 / Mammalian sirtuins: biological insights and disease relevance / Annu Rev Pathol 5 : 253 ~ 295
Imai S / 2000 / Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase / Nature 403 : 795 ~ 800
Kim JJ / 2012 / The role of SIRT1 on angiogenic and odontogenic potential in human dental pulp cells / J Endod 38 : 899 ~ 906
Guarente L / 2013 / Calorie restriction and sirtuins revisited / Genes Dev 27 : 2072 ~ 2085
Haigis MC / 2010 / Mammalian sirtuins: biological insights and disease relevance / Annu Rev Pathol 5 : 253 ~ 295
Imai S / 2000 / Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase / Nature 403 : 795 ~ 800
Kim JJ / 2012 / The role of SIRT1 on angiogenic and odontogenic potential in human dental pulp cells / J Endod 38 : 899 ~ 906
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