지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 145 - 153 (9page)
이용수
초록· 키워드
오류제보하기
In this study, we developed a loop-mediated isothermal amplification (LAMP) with hydroxynaphtol blue dye (HNB) for rapid and direct visual detection of porcine circovirus 2 DNA with high sensitivity and specificity. The LAMP was completed in 40 min at 63oC, and the results of the LAMP can be con-firmed by naked eye without any detection process. The sensitivity of the LAMP was 10-fold higher than that of the commercial PCR (cPCR) and real time PCR (rPCR) previously reported. In clinical application, the PCV2 detection rate of the LAMP was the same on porcine tissue samples (75.0%, 36/48) between porcine blood samples (75.0%, 39/52). The PCV2 detection rate (75.0%) of LAMP was higher than those of the cPCR and rPCR (67.3%, 35/52) in blood samples. In conclusion, the LAMP developed in the study could be an useful alternative method for the detection of PCV2 in the swine disease diagnostic laboratories.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Brunborg IM / 2004 / Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting s
Chae C / 2012 / Porcine circovirus type 2 and its associated diseases in Korea / Virus Res 164 : 107 ~ 113
Chen HT / 2008 / Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification / J Virol Methods 149 : 264 ~ 268
Cho HS / 2005 / Detection of canine distemper virus in blood samples by reverse transcription loop-mediated isothermal amplification / J Vet Med B 52 : 410 ~ 413
Dhama K / 2014 / Loop-mediated isothermal amplification of DNA(LAMP) : a new diagnostic tool lights the world of diagnosis of animal and human pathogens : a review / Pak J Bi
Chae C / 2012 / Porcine circovirus type 2 and its associated diseases in Korea / Virus Res 164 : 107 ~ 113
Chen HT / 2008 / Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification / J Virol Methods 149 : 264 ~ 268
Cho HS / 2005 / Detection of canine distemper virus in blood samples by reverse transcription loop-mediated isothermal amplification / J Vet Med B 52 : 410 ~ 413
Dhama K / 2014 / Loop-mediated isothermal amplification of DNA(LAMP) : a new diagnostic tool lights the world of diagnosis of animal and human pathogens : a review / Pak J Bi
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
등온증폭법을 이용한 돼지인플루엔자바이러스 신속 진단법 개발
한국가축위생학회지
2015 .01
-
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Journal of Veterinary Science
2017 .06
-
제주지역의 돼지 써코바이러스 2형 관련 장염에 대한 병리학적 특징
Korean Journal of Veterinary Research(대한수의학회지)
2020 .09
-
Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems
Journal of Veterinary Science
2015 .03
-
경북지역 양돈장의 돼지생식기호흡기증후군, 돼지써코바이러스-2 항체가 조사
한국가축위생학회지
2015 .01
-
Evaluation of formulation and immunogenicity of porcine circovirus type 2d (PCV2d) vaccine for needle-free intradermal route injection
Journal of Veterinary Science
2025 .03
-
Molecular epidemiological surveillance and complete genome analysis of porcine circoviruses in wild boars (Sus scrofa) in Gyeongnam Province, South Korea
Journal of Veterinary Science
2024 .11
-
Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination
Journal of Veterinary Science
2016 .09
-
Use of a multinomial logistic regression model to evaluate risk factors for porcine circovirus type 2 infection on pig farms in the Republic of Korea
예방수의학회지
2017 .09
-
Molecular Diagnosis of Streptococcus pneumoniae in Middle Ear Fluids from Children with Otitis Media with Effusion
Pediatric Infection and Vaccine
2015 .01
-
Genetic variations in open reading frame 2 gene of porcine circovirus type 2 isolated in Korea during 2016-2017
Korean Journal of Veterinary Research(대한수의학회지)
2018 .10
-
Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis
Annals of Laboratory Medicine
2018 .01
-
[Infectious Disease] Efficacy of bivalent vaccines of porcine circovirus type 2 and Mycoplasma hyopneumoniae in specific pathogen-free pigs challenged with porcine circovirus type 2d
Journal of Veterinary Science
2022 .05
-
Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages
Journal of Veterinary Science
2017 .06
-
Detection and genetic characterization of porcine circovirus 3 from aborted fetuses and pigs with respiratory disease in Korea
Journal of Veterinary Science
2018 .09
-
Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction (direct RT-LAMP) for the Detection of Lymph Node Metastasis in Early Breast Cancer
Cancer Research and Treatment
2022 .01
-
Simple and Rapid Detection of Vancomycin-Resistance Gene from Enterococci by Loop-Mediated Isothermal Amplification
대한의생명과학회지
2020 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0