Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology

Esmatabadi, Mohammad javad Dehghan; Bozorgmehr, Ali; zadeh, Hesam Motaleb; Bodaghabadi, Narges; Farhangi, Baharak; Babashah, Sadegh; Sadeghizadeh, Majid

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자료유형: 학술저널

저자정보: Esmatabadi, Mohammad javad Dehghan (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University) Bozorgmehr, Ali (Department of Neuroscience, Iran University of Medical Science) zadeh, Hesam Motaleb (Department of Genetics, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University) Bodaghabadi, Narges (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University) Farhangi, Baharak (Department of Genetics, Faculty of Biology, University of Guilan) Babashah, Sadegh (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University) Sadeghizadeh, Majid (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제17호

발행연도: 2015.1

수록면: 7,409 - 7,414 (6page)

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Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity. However, evaluation of reaction accuracy or quantitative inspection make it necessary to append other procedures to scrutinize the amplified products. Hitherto, various techniques such as turbidity assessment in the reaction vessel, post-reaction agarose gel electrophoresis, use of intercalating fluorescent dyes, real-time turbidimetry, addition of cationic polymers to the reaction mixture, polyacrylamide gel-based microchambers, lateral flow dipsticks, fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays and nanoparticle-based colorimetric tests have been utilized for this purpose. In this paper, we reviewed the best-known techniques for evaluation of LAMP amplicons and their applications in molecular biology beside their advantages and deficiencies. Regarding the properties of each technique, the development of innovative prompt, cost-effective and precise molecular detection methods for application in the broad field of cancer research may be feasible.

#LAMP #isothermal conditions #evaluation of reaction accuracy #quantitative inspections #various techniques

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