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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 323 - 330 (8page)
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Estrogen may promote osteoblast/osteocyte viability by limiting apoptotic cell death. We hypothesize that hsp27 is an estrogen- regulated protein that can promote osteoblast viability by increasing osteoblast resistance to apoptosis. The purpose of this study was to determine the effect of estrogen treatment and heat shock on TNF –induced apoptosis in the MC3T3-E1 cell line. Cells were treated with 0 – 100 nM 17 estradiol (or ICI 182780) for 0 – 24 hours before heat shock. After recovery, apoptosis was induced by treatment with 0 – 10 ng/ml TNF. Hsp levels were evaluated by Northern and Western analysis using hsp27, hsp47, hsp70c and hsp70i – specific reagents. Apoptosis was revealed by in situ labeling with Terminal Deoxyribonucleotide Transferase (TUNEL). A 5 – fold increase in hsp27 protein and mRNA was noted after 5 hours of treatment with 10–20 nM 17 estradiol prior to heat shock. Increased abundance of hsp47, hsp70c or hsp70i was not observed. TUNEL indicated that estrogen treatment also reduced (50%) MC3T3-E1 cell susceptibility to TNF – induced apoptosis. Treatment with hsp27-specific antisense oligonucleotides prevented hsp27 protein expression and abolished the protective effects of heat shock and estrogen treatment on TNF – induced apoptosis. Hsp27 is a determinant of osteoblast apoptosis, and estrogen treatment increases hsp27 levels in cultured osteoblastic cells. Hsp27 contributes to the control of osteoblast apoptosis and may be manipulated by estrogenic or alternative pathways for the improvement of bone mass.
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참고문헌 (27)
참고문헌 신청
[학술지(정기간행물
/ / Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : 4350 ~ 4354
/ / Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987 : 156 ~ 159
/ / Stein GS. Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells. J. Cell Biochem. 1992 : 277 ~ 287
/ / Uoshima K. Differential estrogenic regulation of small M heat shock protein expression in osteoblasts. J. Biol. Chem. 1994 : 7869 ~ 7873
/ / Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : 4350 ~ 4354
/ / Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987 : 156 ~ 159
/ / Stein GS. Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells. J. Cell Biochem. 1992 : 277 ~ 287
/ / Uoshima K. Differential estrogenic regulation of small M heat shock protein expression in osteoblasts. J. Biol. Chem. 1994 : 7869 ~ 7873
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