Coffee is a complex mixture of chemicals that provides significant amounts of caffeine and polyphenols such as chlorogenic acid. Several studies have shown that drinking of filtered coffee extract (FCE) may prevent cardiovascular disease. The present studies examined that intraduodenal infusion of FCE would lower the lymphatic absorption of cholesterol and fat and also that FCE feeding would affect fat accumulation in rats. In study 1, male Sprague-Dawley rats were housed singly in an animal room and had free access to deionized water and a nutritionally sufficient AIN-93G. At approximately 350 g, rats were starved for 16 h and cannulated with a lymph cannula in the mesenteric lymph duct. Rats were infused at 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing 33.3 kBq cholesterol labeled with 14C-cholesterol, 20.7 μmol of cholesterol, 396.0 μmol of Na-taurocholate, 3.1 μmol ?-tocopherol, 452.0 μmol of triolein and 75.4 μmol retinol without (control group) or with 10.0 mL FCE (FCE group) in PBS buffer (pH, 6.4). Lymph was collected hourly for 8 h. Lymph flow for 8 h was significantly decreased in rats infused with FCE (12.56 ± 3.93 mL) than in that infused with no FCE (20.96 ± 2.52 mL). The infusion of FCE lowered the lymphatic absorption of 14C-cholesterol (25.01 ± 5.75% dose/8 h) compared with control (38.16 ± 3.18% dose/8 h). Also, FCE markedly lowered total fatty acids (control group, 905.57 ± 78.64 ㎛ol/8 h vs. FCE group, 742.27 ± 134.45 ㎛ol/8 h) and oleic acid (control group, 738.94 ± 58.53 ㎛ol/8 h; FCE group, 7593.15 ± 117.05 ㎛ol/8 h) absorption. The results indicate that FCE has an inhibitory effect on the intestinal absorption of 14C-cholesterol in rats. Study 2 was designed to investigate whether FCE could lower lipid levels in organ in rats with a high-fat diet. Initially, male Sprague-Dawley rats were accommodated individually in a stainless steel cage and fed AIN-93G diet supplemented with 3% lard, 0.1% cholic acid and 0.2% cholesterol for 4 weeks. After 4 weeks, rats were weight-matched and assigned to the following two groups: 1) control rats fed standard AIN-93G diet alone, 2) FCE group fed AIN-93G diet supplemented with FCE powder (213 mg/20 g diet) for 4 week. At the 4th week, body and organ weights of FCE group were significantly lower than those of the control. Average liver weights were 18.08 ± 1.79 (control group) and 14.15 ± 2.54 (CFE group). Especially, FCE feeding resulted in reductions in liver cholesteryl ester and triglyceride lipid fractions. Also, FCE markedly decreased average epididymal fat tissue (control group, 14.24 ± 2.78 g; FCE group, 9.18 ± 2.07 g)and retroperitoneal fat tissue weight (control group, 12.58 ± 2.42 g; FCE group, 7.95 ± 2.67 g) weight, suggesting that FCE may have an inhibitory effect on fat storage in rats. In summary, the findings provide evidence that FCE effectively lowers the lymphatic absorption of 14C-cholesterol and also inhibits fat accumulation. This study indicate that FCE could be used as an effective dietary means of lowering fat absorption and fat storge in adipose tissues, thereby preventing cardiovascular disease.