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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 96 - 102 (7page)
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초록· 키워드
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Background: Korean ginseng, Panax ginseng Meyer, has been used as a traditional oriental medicine to treat illness and promote health for several thousand years. Ginsenosides are the main constituents for the pharmacological effects of P. ginseng. Since several ginsenosides, including ginsenoside (G)-Rg3 and G-Rp1, have reported antiplatelet activity, here we investigate the ability of G-Rp4 to modulate adenosine diphosphate (ADP)-induced platelet aggregation. The ginsenoside Rp4, a similar chemical structure of GRp1, was prepared from G-Rg1 by chemical modification.
Methods: To examine the effects of G-Rp4 on platelet activation, we performed several experiments, including antiplatelet ability, the modulation of intracellular calcium concentration, and P-selectin expression. In addition, we examined the activation of integrin αIIbβ3 and the phosphorylation of signaling molecules using fibrinogen binding assay and immunoblotting in rat washed platelets.
Results: G-Rp4 inhibited ADP-induced platelet aggregation in a dose-dependent manner. We found that G-Rp4 decreased calcium mobilization and P-selectin expression in ADP-activated platelets. Moreover, fibrinogen binding to integrin αIIbβ3 by ADP was attenuated in G-Rp4-treated platelets. G-Rp4 significantly attenuated phosphorylation of extracellular signal-regulated protein kinases 1 and 2, p38, and c-Jun N-terminal kinase, as well as protein kinase B, phosphatidylinositol 3-kinase, and phospholipase C-γ phosphorylations.
Conclusion: G-Rp4 significantly inhibited ADP-induced platelet aggregation and this is mediated via modulating the intracellular signaling molecules. These results indicate that G-Rp4 could be a potential candidate as a therapeutic agent against platelet-related cardiovascular diseases.
Methods: To examine the effects of G-Rp4 on platelet activation, we performed several experiments, including antiplatelet ability, the modulation of intracellular calcium concentration, and P-selectin expression. In addition, we examined the activation of integrin αIIbβ3 and the phosphorylation of signaling molecules using fibrinogen binding assay and immunoblotting in rat washed platelets.
Results: G-Rp4 inhibited ADP-induced platelet aggregation in a dose-dependent manner. We found that G-Rp4 decreased calcium mobilization and P-selectin expression in ADP-activated platelets. Moreover, fibrinogen binding to integrin αIIbβ3 by ADP was attenuated in G-Rp4-treated platelets. G-Rp4 significantly attenuated phosphorylation of extracellular signal-regulated protein kinases 1 and 2, p38, and c-Jun N-terminal kinase, as well as protein kinase B, phosphatidylinositol 3-kinase, and phospholipase C-γ phosphorylations.
Conclusion: G-Rp4 significantly inhibited ADP-induced platelet aggregation and this is mediated via modulating the intracellular signaling molecules. These results indicate that G-Rp4 could be a potential candidate as a therapeutic agent against platelet-related cardiovascular diseases.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (46)
참고문헌 신청
[학술지(정기간행물)] - Reddy KS / 1998 / Emerging epidemic of cardiovascular disease in developing countries / Circulation 97 : 596 ~ 601
[학술지(정기간행물)] - McKeigue PM / 1991 / Relation of central obesity and insulin resistance with high diabetes prevalence and cardiovascular risk in South Asians / Lancet 337 : 382 ~ 386
[학술지(정기간행물)] - Davi G / 2007 / Platelet activation and atherothrombosis / N Engl J Med 357 : 2482 ~ 2494
[학술지(정기간행물)] - Furie B / 2008 / Mechanisms of thrombus formation / N Engl J Med 359 : 938 ~ 949
[학술지(정기간행물)] - Adam F / 2008 / Mitogen-activated protein kinases in hemostasis and thrombosis / J Thromb Haemost 6 : 2007 ~ 2016
[학술지(정기간행물)] - McKeigue PM / 1991 / Relation of central obesity and insulin resistance with high diabetes prevalence and cardiovascular risk in South Asians / Lancet 337 : 382 ~ 386
[학술지(정기간행물)] - Davi G / 2007 / Platelet activation and atherothrombosis / N Engl J Med 357 : 2482 ~ 2494
[학술지(정기간행물)] - Furie B / 2008 / Mechanisms of thrombus formation / N Engl J Med 359 : 938 ~ 949
[학술지(정기간행물)] - Adam F / 2008 / Mitogen-activated protein kinases in hemostasis and thrombosis / J Thromb Haemost 6 : 2007 ~ 2016
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UCI(KEPA) : I410-ECN-0101-2018-524-001598734