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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 62 - 67 (6page)
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초록· 키워드
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Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide (H2O2) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.
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참고문헌 (23)
참고문헌 신청
Singh N / 2004 / Free radical scavenging activity of an aqueous extract of potato peel / Food Chemistry 85:611~616
Bayati S / 2011 / Antioxidant and free radical scavenging potential of yakuchinone B derivatives in reduction of lipofuscin formation using H2O2-treated neuroblastoma cells / Iran Biomed J 15:134~142
Kumarappan CT / 2012 / Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens / Saudi J Biol Sci 19:349~355
Debnath T / 2013 / Anti-oxidant and anti-inflammatory activities of Inonotus obliquus and germinated brown rice extracts / Molecules 18:9293~9304
Mahakunakorn P / 2004 / Antioxidant and free radical-scavenging activity of Choto-san and its related constituents / Biol Pharm Bull 27:38~46
Bayati S / 2011 / Antioxidant and free radical scavenging potential of yakuchinone B derivatives in reduction of lipofuscin formation using H2O2-treated neuroblastoma cells / Iran Biomed J 15:134~142
Kumarappan CT / 2012 / Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens / Saudi J Biol Sci 19:349~355
Debnath T / 2013 / Anti-oxidant and anti-inflammatory activities of Inonotus obliquus and germinated brown rice extracts / Molecules 18:9293~9304
Mahakunakorn P / 2004 / Antioxidant and free radical-scavenging activity of Choto-san and its related constituents / Biol Pharm Bull 27:38~46
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