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자료유형
학술저널
저자정보
이여진 (조선대학교) 양승화 (섬섬바이오) 최문희 (섬섬바이오) 신현재 (조선대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.40 No.1(Wn.192)
발행연도
2025.3
수록면
87 - 98 (12page)

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초록· 키워드

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Anthriscus sylvestris leaves are rich in polyphenols known for their anti-inflammatory, antioxidant, and anticancer properties. However, research on the skin-whitening effects of A. sylvestris leaves remains limited. This study aimed to enhance the bioactivity of A. sylvestris leaves through fermentation using two strains of Lactobacillus, and to evaluate their antioxidant and skin-whitening effects, along with the changes in active compound content before and after fermentation. The pre-fermentation extract was prepared via hot water extraction, and fermentation was carried out using Lactobacillus plantarum and Lactobacillus acidophilus. The L. plantarum-fermented extract exhibited stronger antioxidant and whitening activities than the L. acidophilus-fermented extract, and both showed higher activity than the hot water extract alone. The IC₅₀ values for DPPH and ABTS radical scavenging activities of the L. plantarum-fermented extract were 258.47 μg/mL and 174.17 μg/mL, respectively, representing approximately 1.89-fold higher activity than the hot water extract. HPLC and LC-MS/MS analyses revealed increasedlevels of protocatechuic acid, caffeic acid, rutin, and quercetin after fermentation, while levels of ferulic acid and chlorogenic acid decreased. In the tyrosinase inhibition assay, the L. plantarum-fermented extract showed an IC₅₀ value of 334.68 μg/mL, which was approximately 2.42 times more effective than the hot water extract. Additionally, melanin content and melanogenesis-related protein expression analyses confirmed a 46.86% reduction in melanin production at 100 μg/mL, along with suppressed expression of TYR, TRP-1, and TRP-2 proteins.

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Abstract
1. Introduction
2. MATERIALS AND METHOD
3. Conclusions and Prospects
4. CONCLUSION
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